STANDARD OPERATING PROCEDURES FOR ASO TITRE

Incomplete

1. SPECIMEN:

1. Plasma or Serum

2. EQUIPMENTS & REAGENTS

1. Materials:
   1. Streptolysin O buffer
   2. Streptolysin O reagent
   3. ASO Standard
   4. 5% sheep red cells (Or human group O, Rh negative red blood cells. 5 mL washed packed cells in 95 mL of SLO buffer.)
   5. 37° C heat block or water bath 6. Four 2 mL serologic pipette, (can use 1 mL serologic pipettes)
   6. Test tube rack
   7. Nineteen 12x75 mm tubes
   8. Three 16x150 mm tubes
   9. Patient serum specimen
   10. Timer

3. PROCEDURE:

1. Consecutively number fourteen (14) 12x75 mm tubes.
2. Maintain patient identity by placing their initials on the tubes.
3. Label five (5) 12x75 mm tubes S3 through S7. (These are the Standard’s tubes and should be placed in the rack behind the patient’s 3-7 tubes.
4. In the three 16x150 mm tubes, prepare dilutions of the patient’s serum as follows: (be sure to mix each, before pipetting to the next)
   1. Tube marked: 1:10 = 0.5 mL patient serum + 4.5 mL SLO buffer.
   2. Tube marked: 1:100 = 1.0 mL of the 1:10 dilution + 9.0 mL SLO buffer.
   3. Tube marked: 1:500 = 2.0 mL of the 1:100 dilution + 8.0 mL SLO buffer.
5. Set up and perform the test according to the product insert. The Standard (treated as a 1:100 dilution) is set up in tubes S3 - S7 only.
6. Because it is oxygen label and will lose its reactivity in a short time period, the Streptolysin O reagent must be added to tubes within 15 minutes after the reagent is prepared.
7. Prepare lab report of the observations.