STANDARD OPERATING PROCEDURES FOR URINE BILE PIGMENT

1. SPECIMEN:

1. Fresh urine sample

2. EQUIPMENT & REAGENTS

1. Reagent:
   1. Rapid Bile Pigment Reagent

3. PROCEDURE (GMELIN'S TEST):

1. Fill small test tube (75 x 10 mm) with approximately 2 mL fresh urine.
2. Overlap slowly about four drop of Rapid Bile pigment reagent over the sample (do not mix).
3. Wait for1 minute.
4. Presence of bile pigment indicates a green colored ring of biliverdin in between the reagent and sample phase.
5. Run a non negative and positive sample to compare the result
6. Prepare lab report of the observations.